FastQCFastQC Report
Sun 14 Apr 2019
SRR6575689_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575689_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3121506
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA161490.5173464347017113No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC90580.2901804449518918No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC79480.2546206862969349No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC58610.18776193286189422No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG50650.16226142124987106No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC48060.1539641442303811No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46240.1481336252437125No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA39860.12769477297176426No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG38970.12484358511564611No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA38510.12336993745967492No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT37120.11891695867315329No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG36910.11824420648238383No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA36650.11741127519857401No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC36320.11635409318450772No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC34900.11180500694216189No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT34860.11167686366772961No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT32010.10254665536442986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21100.061.3742031
GTATCAA86850.054.3834421
ATCAACG103500.044.0854453
TCAACGC105000.043.41094
CAACGCA106300.043.1010365
TATCAAC108900.042.2897832
AACGCAG111100.041.3657726
GGTAATC14250.035.9689068
ACGCAGA130350.035.0411647
AAGGGTA14950.034.575455
CGCAGAG135250.033.759628
GTACATG165100.032.5442431
GCAGAGT142900.032.018159
TACATGG168100.031.8694362
ACATGGG167800.031.5328183
TATAGCG600.00416490931.328962
AGGTATA28100.031.1044856
TAGGTAT28950.030.1912255
CTTAGGT28800.030.0221443
TTAGGTA29700.029.587044
Produced by FastQC (version 0.11.2)