FastQCFastQC Report
Sun 14 Apr 2019
SRR6575688_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575688_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312641
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA161140.4864396715490752No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC90690.27376947879350644No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC82620.2494082516034789No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC59230.17879993636497282No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG52060.15715557466082197No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC50530.15253690333483164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48850.1474654210945285No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA39640.11966283095572385No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA38850.11727802680700987No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT38240.11543659575547124No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG38210.1153460335726087No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA37870.11431966216683305No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC36470.11009342696658045No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG35790.10804068415502918No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC35050.1058068169777528No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT34990.10562569261202769No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT34580.10438800944623941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.062.6255071
GTATCAA90850.054.2611471
ATCAACG113100.043.3024253
TCAACGC115450.042.705984
CAACGCA115850.042.6795925
TATCAAC117400.041.876532
AACGCAG121150.040.8124736
ACGCAGA143250.034.3827827
GGTAATC14100.034.3314748
CGCAGAG148900.033.0781338
GTACATG183500.032.2065121
ACATGGG185650.031.5956123
TACATGG187600.031.5177332
GCAGAGT156950.031.1719369
AAGGGTA15400.031.1300665
TAGGTAT31200.029.0738985
ACCTAAG54900.028.7577341
AGGTATA31400.028.5893526
GTACTAG2650.028.454851
CATGGGG88000.028.3608324
Produced by FastQC (version 0.11.2)