FastQCFastQC Report
Sun 14 Apr 2019
SRR6575685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305363
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27720.21235472431806326No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17730.1358242879566833No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17650.13521143160944502No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17360.13298982735070627No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16110.1234139469251082No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14200.10878200163479432No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13920.10663700441946034No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13670.10472182833434072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.055.97671
GTATCAA21100.055.0428731
TCAACGC26800.042.778494
ATCAACG27550.041.4433753
CAACGCA27700.041.218955
AACGCAG29600.039.049356
TATCAAC34900.033.41282
ACGCAGA36500.031.409967
GTACATG45350.030.586611
ACCTAAG12350.030.458581
TACATGG45550.030.4523092
GTCTTAG8500.030.4249841
ACATGGG47150.029.297873
CGCAGAG39600.028.9510978
CTAAGAC14450.028.6144373
CTTAGGT7600.028.4390243
TAAGACA14800.027.9377464
TAGGTAT7000.027.5204945
GCAGAGT42650.026.9909069
TAACGTT350.00885069726.84926442-43
Produced by FastQC (version 0.11.2)