FastQCFastQC Report
Sun 14 Apr 2019
SRR6575684_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575684_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402032
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA30210.21547297065972817No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18300.13052483823479066No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17590.12546075981147364No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17190.12260775788284432No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17030.1214665571113926No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14720.10499047097355839No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG14480.1032786698163808No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14280.10185216885206613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22350.054.5974851
GGTATCA7350.051.9216041
TCAACGC28800.041.2938964
ATCAACG29800.040.0659373
CAACGCA30000.039.7974135
AACGCAG30550.039.234796
ACGCATA550.00271584634.185326
TATCAAC37200.032.4749152
TACATGG43750.032.125582
GTACATG44600.031.9023361
ACGCAGA37850.031.5390227
ACATGGG46400.030.5947443
CGCAGAG40150.029.7323068
CATGGGG27750.028.9661624
ACCTAAG12050.028.9331071
GTCTTAG10150.028.3148461
CTTAGGT6850.028.1352713
CTAAGAC14300.027.6122063
TAAGACA16500.027.3492324
TAGGTAT6600.027.0633775
Produced by FastQC (version 0.11.2)