FastQCFastQC Report
Sun 14 Apr 2019
SRR6575677_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575677_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047357
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA83440.27381104347144103No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC52020.17070530298878667No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC43640.14320606348386486No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC35220.11557556269252338No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA32610.10701076375363963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48650.051.8470341
GGTATCA13150.051.6285171
TCAACGC56750.043.3179934
ATCAACG56500.043.0951163
CAACGCA57850.041.9255565
AACGCAG60100.040.2777566
TATCAAC70450.034.4950142
ACGCAGA74650.032.0462887
GTACATG97450.031.3506181
TACATGG97450.030.7741182
ACATGGG101600.029.7484283
CGCAGAG81100.029.4396468
GCAGAGT84850.027.8052869
TAGGTAT21550.026.8281275
AGGTATA21750.026.5814326
TTAGGTA21550.026.3918974
GTCTTAG26200.026.0927371
CTTAGGT21850.025.8152643
CATGGGG64250.025.3125254
ACCTAAG36000.025.1450021
Produced by FastQC (version 0.11.2)