FastQCFastQC Report
Sun 14 Apr 2019
SRR6575676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088200
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA71910.3443635667081697No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC48040.23005459247198543No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC42590.2039555598122785No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC30150.14438272196149793No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA29110.13940235609615936No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC28670.1372952782300546No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT27590.13212335983143378No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26530.12704721769945407No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT25210.12072598410113974No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA25020.11981610956804903No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG23920.1145484149027871No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT23490.11248922517000289No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG23330.11172301503687386No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG23060.11043003543721866No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22620.10832295757111388No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22600.10822718130447276No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA22250.10655109663825305No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT21120.10113973757302941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9450.053.7225951
GTATCAA33850.046.3823551
ATCAACG36850.041.950413
TCAACGC37050.041.5971374
CAACGCA38800.039.7209785
AACGCAG39950.038.9304126
GTCTTAG18150.036.5180471
AGGTATA15500.035.164476
TAGGTAT15700.034.716525
CTTAGGT16300.034.30343
TTAGGTA16700.033.763124
GTACATG66700.032.8416631
TACATGG67500.032.3835642
ACATGGG69650.031.5720333
TATCAAC50500.030.904532
GGTATAG18000.030.5415557
TCTTAGG21400.029.8744952
GGTAATC7400.029.8431028
GTATAGT18600.029.8089648
ACGCAGA52700.029.5117657
Produced by FastQC (version 0.11.2)