FastQCFastQC Report
Sun 14 Apr 2019
SRR6575671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338093
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA439241.3158411104783478No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC381101.1416698096787596No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC325210.9742388842971121No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC183370.549325617950129No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT179840.5387507178499821No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG171500.5137663929674817No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG157640.47224568039296694No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA130300.39034262975896716No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA130020.38950382748473455No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC126340.37847956902339147No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT125030.374555172668946No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA120920.36224275357217434No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT116990.35046956450883787No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA113280.33935543437525556No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT112500.33701877089703614No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC98810.29600733113187677No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT96190.28815853842298583No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT95140.2850130298946135No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT94880.2842341420685403No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG89330.26760788270428654No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT86280.2584709293599669No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA83820.2511014522363517No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT72870.2182982918690402No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC70100.21000014079895318No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG69820.20916133852472057No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA69670.2087119801635245No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG69470.2081128356819298No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA67860.2032897226050922No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC67670.2027205353475772No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA67630.20260070645125827No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG67390.2018817330733446No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA65000.19472195651828753No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG64210.19235533581598835No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT63990.19169627688623417No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG62900.18843093946154288No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA62390.1869031210334763No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA60690.1818103929399211No Hit
GTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT60430.18103150511384794No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC60250.18049227508041268No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA60080.17998300227105715No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA59980.1796834300302598No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA59310.17767629601691745No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC58960.17662779317412666No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT58810.1761784348129306No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA58450.1750999747460601No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC57800.17315275518087725No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT55980.16770054039836518No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG55130.16515417635158755No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG53610.16060067829146762No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT53260.15955217544867684No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT51440.1540999606661648No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA51240.15350081618457004No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC51010.1528118000307361No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG50250.15053505100067613No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC49970.14969624872644352No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT49640.1487076603318122No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC49210.14741949969638354No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC47340.1418174987934728No Hit
GAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAG45230.13549652451264838No Hit
CTCTTACAGTATGGAATTTAAGAACTAAGGAATTCTCCAAGACATGGATT44860.13438810722169814No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA44560.13348939049930605No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG44490.13327968993074787No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT44420.13306998936218972No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA43760.13109281257292713No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA43450.1301641386264553No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC43440.13013418140237554No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT43160.12929537912814293No Hit
CCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCAGAC42950.12866627742246847No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGAACATGGCAAC42890.12848653407799004No Hit
ACCATAGATAATTCGGAGCACTCTTCATCTCTTACAGTATGGAATTTAAG42780.12815700461311294No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT42020.12588025558305296No Hit
ATTCCATACTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTG41140.12324401986403613No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCACCTCATTAACGAAA41050.12297440484731852No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA40960.12270478983060087No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC40770.1221356025730859No Hit
GGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGC40430.12111705695437484No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC40240.12054786969685985No Hit
CAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTG39620.11869052180391619No Hit
ATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTG39390.11800150565008224No Hit
GTGTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTA39380.1179715484260025No Hit
GGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGA38770.11614415775713857No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC38320.11479608267355045No Hit
GTCTTGGAGAATTCCTTAGTTCTTAAATTCCATACTGTAAGAGATGAAGA38070.11404715207155702No Hit
CCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGC37540.11245941919533098No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA37470.11224971862677283No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG37240.1115607024729389No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGACT37060.11102147243950364No Hit
GGGTATATCAAAGAAAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT36890.1105121996301481No Hit
TAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTT35990.10781604946297181No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC35470.10625827381082552No Hit
GTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAG35100.10514985651987527No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA34620.10371190976404793No Hit
CCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAAC34510.10338238029917082No Hit
GTATCAACGCAGAGTACATGGGGGAGTGTAGCCACGATCACAAGAAAGAC34040.1019743907674232No Hit
GGCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAAT33960.1017347329747853No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATTGGC33800.10125541738950954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14700.065.880481
ATCAACG78300.048.3796843
TCAACGC79200.047.8291974
CAACGCA81000.046.94045
GTGCAAG24550.045.767091
AACGCAG83300.045.700746
GTATCAA86250.045.512871
TATCAAC84400.045.3964652
AAGGGTA24750.044.814655
AGGGTAA27150.041.0262456
GGTAATC29300.037.6949778
ACGCAGA101000.037.645277
TGCAAGG28750.037.2824022
CGCAGAG105650.035.9883778
GTACATG145400.035.9540021
TACATGG149900.034.937392
ACATGGG149350.034.7104343
GCAGAGT111350.034.2305539
GTAATCA33800.034.0669029
TAGGTAT94350.033.922555
Produced by FastQC (version 0.11.2)