FastQCFastQC Report
Sun 14 Apr 2019
SRR6575670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390807
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7100.1816753538191486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4330.11079637775167794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12300.040.5430341
GGAGCGT550.002719067734.1709336
AACGCAG15250.033.582746
TACGATT352.1884555E-433.56073482-83
TCAACGC15750.032.222434
ATCAACG15950.031.8183863
GTACATG23050.031.6356141
CAACGCA16100.031.5179085
CGATTAA300.00414973231.34942284-85
TACATGG23400.030.957422
ACATGGG23150.030.8537313
CATGGGG9850.029.5781214
ACGCAGA17450.029.3488167
GCGTAGA650.006120435428.9509051
TATCAAC17400.028.9264012
CGCAGAG18350.028.1654138
ACTGCGC1057.425342E-526.8485878
ATACTAA700.00884274326.8485876
ATACGAT350.00884805826.84858782-83
GGGTACC2700.026.1027957
Produced by FastQC (version 0.11.2)