FastQCFastQC Report
Sun 14 Apr 2019
SRR6575668_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575668_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935163
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA232540.792255830425772No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC150250.5118966135781897No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC115520.39357269085226276No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC75270.2564423168321487No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG70450.24002074160787662No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC69850.23797656211937804No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT61730.2103119997083637No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA60660.20666654628720788No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT60490.20608736209879996No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT55370.18864369713027862No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA52780.17981965567159303No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT52070.17740070994353635No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG51200.17443664968521339No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC50180.17096154455476578No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC49480.16857666848485076No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT43510.14823708257428975No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA42430.14455755949499227No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG42140.14356953940888462No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT40360.13750514025967212No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT39390.13420038341993273No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA38640.13164515905930949No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA38200.1301460941010772No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG37470.12765900905673722No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT35120.11965263939345107No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG34850.1187327586236267No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA34840.11869868896548505No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA34790.11852834067477684No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA34120.11624567357928675No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG33990.11580276802344537No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT30820.10500268639254448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.058.671451
GTATCAA72300.049.9349481
ATCAACG75000.046.814523
TCAACGC74800.046.751184
TATCAAC76250.046.293642
CAACGCA77750.044.9773375
AACGCAG80550.043.297186
ACGCAGA96450.036.1552287
GGTAATC12850.035.476888
AAGGGTA13250.035.473615
CGCAGAG100350.034.7506838
GTCTTAG50100.032.6441881
AGGTATA46050.032.559846
TAGGTAT46050.032.559845
TTAGGTA46200.032.454134
CTTAGGT46750.032.072313
GCAGAGT109750.031.9027849
GTACATG135600.031.3516881
GTGCAAG21300.030.7573781
ACCTAAG76600.030.5187721
Produced by FastQC (version 0.11.2)