FastQCFastQC Report
Sun 14 Apr 2019
SRR6575664_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575664_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684732
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA457611.2419085024365408No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC300490.8155002860452266No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC249320.6766299421504739No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG156270.4241014000475476No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC147720.40089754153083584No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT125700.34113742871937497No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT116500.31616953417507704No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA116330.31570817090632375No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG113860.3090048340014959No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC112710.3058838471834586No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA110260.2992347883102489No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT105040.2850682220579407No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC101230.27472825703470427No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA95620.2595032691658443No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT95030.2579020672331122No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC91980.24962466741136127No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT90560.2457709271664805No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG87600.23773777848701072No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA80560.21863191135746102No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG74750.20286414317242066No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT73630.1998245734018105No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT73480.19941748816467522No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA73310.19895612489592185No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT71580.1942610751609615No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA70110.19027163983703563No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT69430.18842618676202233No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA68460.18579370222854744No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA66590.18071870627226078No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA64310.17453101066780433No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC60410.16394679450228675No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT59760.16218275847470046No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCACCTCATTAACGAAA58380.15843757429305577No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG57260.1553980045224456No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC56740.15398677570037658No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA56470.15325402227353305No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG55820.15148998624594678No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG55750.15130001313528366No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG54830.14880322368085386No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC51510.1397930704322594No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA51310.139250290116079No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA51010.13843611964180844No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA50840.1379747563730551No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC50750.1377305052307739No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT49850.13528799380796216No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT49160.1334154017171398No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC48860.1326012312428692No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC48540.1317327827369806No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA47900.12999588572520338No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG47870.1299144686777763No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG46730.12682062087554807No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA45930.12464949961082653No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC45810.12432383142111827No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT44880.12179990295087947No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT44380.12044295216042848No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT44240.12006300593910221No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGAACATGGCAAC44080.11962878168615791No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT43770.1187874721960783No Hit
GTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT43390.11775618959533557No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT43160.11713199223172813No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA42560.11550365128318696No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA42220.1145809247456803No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA42130.11433667360339912No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA41390.11232838643353167No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA40520.10996729205814697No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC39650.10760619768276228No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT39620.10752478063533523No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC39100.10611355181326619No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC38610.10478374003862424No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA38440.10432237676987091No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA38410.10424095972244385No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG37460.101662753220587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.067.425681
AAGGGTA21250.046.4476285
GTATCAA108650.045.595451
ATCAACG105150.045.1014983
AGGGTAA22450.044.1742636
TCAACGC107500.044.0712364
GTGCAAG23350.044.0066381
TATCAAC109800.043.4911042
CAACGCA109350.043.282655
GGTAATC23350.042.0656138
AACGCAG113200.041.8105856
GTAATCA28500.036.1137859
TTAGGTA86200.035.7684174
TGCAAGG28100.035.292732
TAGGTAT87500.035.075865
ACGCAGA134600.035.0564777
GTCTTAG90500.035.0000951
AGGTATA88100.034.836986
CTTAGGT89050.034.7297023
CGCAGAG139600.033.7326248
Produced by FastQC (version 0.11.2)