FastQCFastQC Report
Sun 14 Apr 2019
SRR6575659_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575659_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426633
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6810.15962197017108382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.11016494270251012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13500.040.5352551
GAGTCGA500.001701234137.598179
AACGCAG16300.034.5995456
GCGAATT550.002715749434.18015794
TACATGG27150.033.7552642
ATCAACG16350.033.6313933
CAACGCA16650.033.589965
GTACATG27600.033.500841
TCAACGC16550.033.2249684
ACATGGG27900.032.005613
ATGGGTA4200.031.331815
CGCAGAG18900.030.0884868
ACGCAGA19050.029.8515667
ATGGGAG6000.028.9819245
TATCAAC19500.028.4396422
CATGGGT7450.027.757044
TAGCGGG350.008836864526.85583712-13
CATGGGG9850.026.7195134
GCAGAGT21700.026.20619
CATGGGA15300.025.8026684
Produced by FastQC (version 0.11.2)