FastQCFastQC Report
Sun 14 Apr 2019
SRR6575659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426633
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7380.17298239939245205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.11836871503142045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12400.039.4317471
GTACATG25650.036.8419881
TACATGG26150.035.9577642
ACATGGG25900.034.8403973
ATCAACG15000.031.9588033
AACGCAG15100.031.4359116
TCAACGC15500.030.9278744
CAACGCA15350.030.9239235
ATGGGAG6600.028.4837845
GTATAGG1005.3044205E-528.2088641
CATGGGA13750.028.0280444
ACGCAGA16750.027.7780657
TATCAAC17300.027.7196922
CATGGGG9900.027.0595934
CGCAGAG17300.026.8949478
GTCCTAT1501.1564935E-625.0745471
CATGGGT5650.024.9548194
GGGTACC2850.024.7359167
TTAGTAG950.00130352624.7359143
ATGGGTA3100.024.2571585
Produced by FastQC (version 0.11.2)