FastQCFastQC Report
Sun 14 Apr 2019
SRR6575658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402219
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA246511.026176214574941No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC191050.7953063396801041No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC150770.6276280389090253No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC105980.4411754298837866No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG82120.3418505973019113No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA68180.28382091724359854No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG65590.27303921915528934No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC57300.23852945963711053No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA56640.2357819998925993No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA54760.22795590243853706No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG53790.22391796917766446No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC53460.22254423930540887No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT52410.21817328062095923No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT51420.21405209100419237No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA48850.2033536492717775No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT48500.20189666304362758No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT48490.20185503486568043No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG47070.19594383359718662No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT46820.1949031291485081No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA44080.18349700839099184No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT44060.18341375203509755No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT44060.18341375203509755No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT41050.17088367047300848No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC40730.1695515687787No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT40590.16896877428744006No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA40110.16697062174597738No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT38550.1604766259862236No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT37930.1578956789535009No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG36150.15048586327891003No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA36000.14986144060970294No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA35710.1486542234492359No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA35530.1479049162461874No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG35460.14761351900055739No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC35090.14607327641651324No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA34260.14261813764690065No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC34120.14203534315564068No Hit
CTATTATGCTGGCCACTGACAAAAGAGAAAAGATAGAAGAACATGGCAGC33480.13937113976702373No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT33110.13783089718297956No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA32710.13616577006509398No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA31000.1290473516361331No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC30590.12734059634030037No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA30400.1265496609593047No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA29780.12396871392658204No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT29670.12351080396916352No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG29500.12280312494406215No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA29010.12076334422465229No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG28460.1184737944375596No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA28150.11718332092119828No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG27790.11568470651510125No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA27780.11564307833715409No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA27750.11551819380331269No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG27180.11314538766032571No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT26150.10885768533177033No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC25920.10790023723898613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25870.10769209634925042No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT24630.10253020228380509No Hit
GTCTTAAGTATAGTAAATGTATTCGATCCATCATACGTTACAGAATATTC24620.10248857410585796No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG24570.10228043321612226No Hit
CTGTAAGAGATGAAGAGTGCTCGGAATTATCTATGGTTGCTGACAAAACA24400.1015727541910209No Hit
ATATATGCCAGCCTTTTCTGTTTGTTCACCAATCGCAGTCATTTCGGTGC24070.10019902431876526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.062.9153061
GTATCAA54350.050.67681
TCAACGC60500.044.114114
TATCAAC61350.043.8995632
ATCAACG60800.043.8191573
CAACGCA61900.043.1913875
AACGCAG63300.042.533046
GTGCAAG17950.040.0625081
GTACATG106200.037.795881
ACATGGG109250.036.342923
AAGGGTA15250.036.048865
TACATGG111350.035.9219172
ACGCAGA76650.035.0025337
CGCAGAG79100.033.9183858
GGTAATC16400.033.521048
CATGGGG71300.032.8848274
GCAGAGT83700.031.9974889
GTCTTAG45350.031.4033851
TAGGTAT43400.031.2884225
AGGGTAA17950.031.1499986
Produced by FastQC (version 0.11.2)