FastQCFastQC Report
Sun 14 Apr 2019
SRR6575632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3121304
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC73120.23426106524708903No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA57270.1834810066561924No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT54470.17451039693666492No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT52810.16919210688865935No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT49270.15785069317182818No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT47440.15198775896227987No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG43770.14022985265132779No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA43220.13846776859927773No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA41650.13343781957797124No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC40400.12943308309603935No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA40370.12933696942047299No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC37940.12155176169959736No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA37250.11934114716157093No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC36970.1184440861896182No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA35570.11395878132985444No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT34710.1112035226302853No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA34660.11104333317100801No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG34640.11097925738729711No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT33990.10889679441669252No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT32790.10505224739403789No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG32500.10412314853022966No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC31240.1000863741564423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47750.048.4334031
CAACGCA53950.040.410935
ATCAACG54950.039.7610243
GTACTAG15850.038.553811
GTATATA3250.034.712121
AACGCAG68150.033.3007166
GTACATG95900.032.8404771
TACATGG97350.032.1098982
TAGTACT24400.031.7736024
ACGCAGA69750.031.2569127
ACATGGG100250.030.793093
TCAACGC74450.029.6623424
CTAGTAC25750.029.5603853
CGCAGAG74400.029.3033588
GCATATA3700.029.2199441
GTACTGT27500.028.0209886
CCTAGTA27000.027.6813072
CATGGGG72000.027.6697794
TAGGCAT23850.027.3841155
CTGTGCG28250.026.9444249
Produced by FastQC (version 0.11.2)