FastQCFastQC Report
Sun 14 Apr 2019
SRR6575627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3380227
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA432261.2787898564208855No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC260200.7697707875832007No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC252910.7482041886536023No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG164000.4851745163860297No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC162300.48014526835032084No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA120840.35749078390297456No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT116950.34598268104479374No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG109340.323469400132003No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA108060.31968267219923396No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA105460.3119908810857969No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC105250.31136962103432697No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT99480.2942997615248917No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC97390.2881167448221673No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT95030.2811349651961244No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT94370.27918243360579037No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA86740.2566099850690501No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT86300.25530829734216076No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA84250.2492436158873354No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT83360.2466106566215819No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT77860.23033956003546507No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA67060.19838904310272654No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG65430.193566881750841No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA64140.1897505700060972No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC63190.18694010786849521No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT61640.18235461701240774No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA60320.17844955383173972No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG60140.17791704521619406No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA59180.17507699926661732No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG56910.16836147394834727No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG56560.16732604052923072No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC54960.16259263061326948No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA54890.16238554392944615No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC53620.1586283998086519No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG53060.15697170633806545No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG52190.15439791469626152No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC49860.14750488650614293No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA49580.1466765397708497No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA48950.14481275961643997No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG48270.14280106040215643No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC47930.14179521079501464No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA47410.14025685257232726No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG47080.13928058677716024No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT46990.1390143324693874No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC46750.13830432098199322No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC46610.13789014761434662No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT46440.13738722281077573No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA45880.13573052934018928No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT45410.13434009017737567No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA45340.13413300349355237No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT44270.13096753561225327No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT43330.128186657286626No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT43140.12762456485910562No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA43010.12723997530343376No Hit
GTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT42870.12682580193578716No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA42090.12451826460175602No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT41080.12153029959230549No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA40750.12055403379713847No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC40020.11839441552298116No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA39990.11830566408705687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39930.11812816121520833No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT39010.1154064505135306No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG38660.11437101709441408No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT38210.11303974555554996No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA37840.11194514451248391No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC37490.1109097110933674No Hit
GGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGA37280.11028845104189748No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCACCTCATTAACGAAA36990.10943052049462951No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG36960.10934176905870524No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC36740.10869092519526055No Hit
CAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTG36660.10845425469946249No Hit
ATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTG36270.10730048603244692No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC35780.10585087924568379No Hit
CCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCAGAC35060.10372084478350123No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG35020.1036025095356022No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA34600.10235998943266236No Hit
GAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAG34450.101916232253041No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT33880.10022995497047979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.067.6413351
GTGCAAG29500.045.2577821
GTATCAA111350.044.7942351
ATCAACG107350.044.656623
TATCAAC109800.043.7996142
TCAACGC109650.043.719914
CAACGCA110500.043.5112045
AAGGGTA31900.041.989625
AACGCAG115200.041.980176
AGGGTAA33750.039.269626
GGTAATC34700.038.0590678
GTACTAG4400.037.3948971
TGCAAGG35200.037.3948972
ACGCAGA137200.035.1116377
CGCAGAG140650.034.150148
GTACATG178250.033.3888241
GCAAGGG39750.033.2243843
GTAATCA41500.032.955379
TTAGGTA74450.032.7635084
TAGGTAT74700.032.5280275
Produced by FastQC (version 0.11.2)