FastQCFastQC Report
Sun 14 Apr 2019
SRR6575626_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575626_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002784
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19300.19246417972364935No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12420.12385518715894948No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12070.1203649041069662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.10969461020518875No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10910.10879710884896447No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10720.10690238376360214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19650.062.284491
TCAACGC29550.040.87034
CAACGCA30250.040.079895
ATCAACG30350.039.792993
AACGCAG31500.038.936976
GTACATG56150.036.9707221
TACATGG56200.036.707972
ACATGGG56750.036.18663
TATCAAC35750.034.176652
ACGCAGA37950.032.5668837
GGTATCA7750.031.584261
CGCAGAG39700.031.3884038
CATGGGG30150.029.6140964
CATGGGA19650.029.176254
GCAGAGT45150.027.182959
CATGGGT14050.027.0919884
GTATAGG2550.025.8438361
AGAGTAC40650.025.44930610-11
ATGGGTA9850.023.8542615
ATGGGGA16700.023.355755
Produced by FastQC (version 0.11.2)