FastQCFastQC Report
Sun 14 Apr 2019
SRR6575626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002784
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG21850.2178933848166704No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA13240.13203242173788174No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12990.12953936241503655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.12006573698822479No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA11500.11468072885087917No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11230.11198822478220634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20650.058.7348251
TCAACGC28850.041.6940044
CAACGCA29800.040.5225035
ATCAACG30100.039.962523
AACGCAG30000.039.939116
GTACATG54250.035.095491
TACATGG56250.034.1768342
ACGCAGA36000.033.543637
TATCAAC36300.033.1485372
ACATGGG56750.032.9531253
CGCAGAG38300.031.4065768
CATGGGG28650.029.6847484
GTACGTA804.7741283E-429.3816411
GCAGAGT43450.027.5759099
GGTATCA7850.027.5476261
CATGGGA20750.026.7204234
CATGGGT14350.026.1949464
AGAGTAC40100.024.60675610-11
ATAGACG1000.001761315723.4935938
TAATACC2202.4574547E-923.4935914
Produced by FastQC (version 0.11.2)