FastQCFastQC Report
Sun 14 Apr 2019
SRR6575625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2950570
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA114810.38911125646908906No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC73030.24751149777839537No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC63750.2160599477389115No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC41460.1405152224824356No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC40210.13627875291892755No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG35680.1209257872207743No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT29730.10076019209847588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13950.054.9277151
GTATCAA54800.049.4963761
TCAACGC58000.045.934024
ATCAACG59300.044.768563
CAACGCA60750.043.93135
AACGCAG62800.042.946156
TATCAAC69000.038.8332632
ACGCAGA79150.033.8966837
GTCTTAG26000.033.629291
GTACATG112300.033.4042171
TAGGTAT22200.033.229115
TTAGGTA21850.032.6867334
TACATGG113900.032.439712
ACATGGG115100.031.719413
CTTAGGT22850.031.4618763
ACGTCGA600.00416793631.32425594
ACCTAAG37400.031.0458431
AGGTATA23250.030.7179816
CGCAGAG87550.030.4834588
CTAAGAC42500.029.1873283
Produced by FastQC (version 0.11.2)