FastQCFastQC Report
Sun 14 Apr 2019
SRR6575623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071099
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA132520.6398535270404747No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG59070.28521089527830396No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC55060.26584919407522284No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA49720.24006578150054633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.22268370560750597No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC44480.21476520436734314No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC42970.20747438920109565No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA42230.2039014069341929No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC37720.1821255285237451No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC32490.15687323493468924No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG29930.14451264763297167No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT29630.14306414130855163No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA29590.1428710071319623No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG28350.13688384765769285No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA27720.1338419843764108No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT27080.13075183755098138No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT25220.12177109833957721No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT23780.11481826798236106No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA22960.11085901736227965No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA22820.11018304774421696No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC22620.10921737686127027No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA21810.1053064097853362No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC21630.10443730599068418No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA20920.10100917435622345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21900.062.0361861
GTATCAA82050.054.7162251
ATCAACG102000.043.2550243
GTGCAAG15850.042.709511
CAACGCA104100.042.3373075
AAGGGTA16600.041.325295
TATCAAC107500.041.193992
GGTAATC16550.040.8813448
GTACTAG7500.040.7421041
TCAACGC109000.040.4771774
AACGCAG114200.038.922096
AGGGTAA17900.038.061526
ACGCAGA124900.035.249127
GTAATCA20000.035.2388659
CGCAGAG128150.034.3910038
TAGGTAT13150.033.9444665
CTTAGGT13400.033.6618163
GCAGAGT133150.033.1348539
ACCTAAG48500.032.3739781
CAAGGGT20550.032.238774
Produced by FastQC (version 0.11.2)