FastQCFastQC Report
Sun 14 Apr 2019
SRR6575622_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575622_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155998
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA145400.6743976571406838No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG52450.2432748082326607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51390.2383582916125154No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC49760.2307979877532354No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC45330.21025065885960934No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG42030.19494452221198721No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC41750.1936458197085526No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA40510.18789442290762792No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC37300.17300572635039552No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA35740.16577009811697413No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA33260.15426730451512477No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC31360.14545468038467568No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT30440.1411875150162477No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG29560.1371058785768818No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC28430.13186468633087786No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA26900.12476820479425306No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT26240.12170697746472862No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA25170.11674407861231784No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC24390.11312626449560714No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA23610.10950845037889646No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG23300.1080706011786653No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT23250.10783869001733767No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT22910.10626169412030993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.10208729321641301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82700.055.895131
GGTATCA23950.053.9082871
ATCAACG99100.045.3920333
CAACGCA101300.044.4526255
TATCAAC108050.042.1976552
TCAACGC107500.041.9325754
AACGCAG110950.041.2217986
GTACTAG8800.040.1595731
GTGCAAG11900.039.201141
ACGCAGA123200.036.5448077
GGTAATC12150.035.9730728
CGCAGAG127050.035.3634038
AAGGGTA12900.034.980255
GCAGAGT132850.033.7133759
GTAATCA14400.032.636869
TAGGTAT14300.031.5556075
GTACATG137000.031.299061
CAAGGGT15500.031.2353884
TAGGCAT12300.030.954325
TACATGG139250.030.7851312
Produced by FastQC (version 0.11.2)