FastQCFastQC Report
Sun 14 Apr 2019
SRR6575619_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575619_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575563
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC43970.2790748449919172No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT28940.18368037330148018No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC26090.16559160122445118No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA24090.1528977260826765No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA23420.14864527791018195No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG23370.14832793103163758No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT20830.13220670960158368No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC20140.1278273226776714No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT20100.1275734451748359No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT18980.12046487509544208No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA18730.11887814070272024No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18080.11475263128164345No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC17990.1141814069002636No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC17810.11303895813750386No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT17370.11024630560631343No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA16730.10618426556094551No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT15910.10097977675281788No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA15790.1002181442443114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28600.047.378411
CAACGCA32500.040.05225
ATCAACG33000.039.445353
ACCTAAG6450.035.01351
TACATGG46600.033.480812
GTACATG48050.033.096141
ACGCAGA40000.032.6598937
ACATGGG47950.032.145133
GGTATCA14000.031.9263741
TATCAAC43700.031.4011232
GTACTAG9600.030.8761651
AACGCAG45750.030.712146
CGCAGAG42750.030.4703148
CCTAAGA7850.029.3339252
GCAGAGT44700.029.0358729
GTATATA2151.8189894E-1228.448471
CATGGGG38400.028.269024
ATGGGGG25600.027.7183235
TAGTACT14300.027.6040744
TAACCAC3800.027.2062746
Produced by FastQC (version 0.11.2)