FastQCFastQC Report
Sun 14 Apr 2019
SRR6575618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608044
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC50100.3115586389427155No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT30280.18830330513344162No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC27200.1691496003840691No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA26570.16523179713987926No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA26380.16405023743131406No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG25100.15609025623676964No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT23190.14421247179803537No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT20090.1249343923424981No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT19570.12170064998221443No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA19550.12157627527604965No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT19440.1208922143921435No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA19130.11896440644658977No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC18900.11753409732569507No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC18550.11535753996781183No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC17300.10758412083251452No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17290.10752193347943215No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17170.10677568524244362No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA16820.10459912788456037No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT16540.10285788199825378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33550.046.8012121
CAACGCA36750.041.690965
ATCAACG37350.040.51793
GTACTAG9000.032.9079781
ACGCAGA46600.032.6768887
TATCAAC49500.032.2906572
TAGTACT15700.032.030654
AACGCAG51050.031.7617936
TACATGG54150.031.427752
CGCAGAG49050.031.0447128
GTACATG55550.030.8895781
CTAACTG6150.030.5679634
ACATGGG55600.030.09243
CTGTGCG17000.029.5812479
GTACTGT17650.029.5569696
GCAGAGT52200.029.4414279
GGTATCA15850.029.3635861
CTAGTAC17900.028.8816013
TCAACGC54000.028.6341154
CGGGCGT10900.028.457656
Produced by FastQC (version 0.11.2)