FastQCFastQC Report
Sun 14 Apr 2019
SRR6575615_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575615_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111827
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20000.1798841006739358No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12830.1153956505823298No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12400.11152814241784019No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.10676121374998089No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA11500.10343335788751307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21500.051.533371
GTACATG57050.035.4536361
TCAACGC30950.035.3838044
TACATGG57450.034.9339032
CAACGCA31650.034.749735
ATCAACG31550.034.7108963
AACGCAG32550.034.3664976
GGTATCA10150.033.9090771
ACATGGG58900.033.7547073
CATGGGG30400.029.5303844
TATACCG1452.6591806E-829.1731825
ACCGTAT650.00615599528.9212388
ACGCAGA38450.028.601567
TATCAAC38500.028.4449042
CGCAGAG40350.027.1382968
CATGGGA22350.026.9179334
CATGGGT14250.025.0673274
GCAGAGT45000.024.6473189
ATGGGGA16000.024.3818915
ATGGGAG12100.024.0832775
Produced by FastQC (version 0.11.2)