FastQCFastQC Report
Sun 14 Apr 2019
SRR6575615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111827
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG21690.19508430718088335No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA13080.117644201840754No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12790.11503588238098193No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12480.11224767882053593No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA11610.10442272044121972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.10325347378683915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22800.054.4380071
TCAACGC30000.040.261394
CAACGCA30650.039.407565
ATCAACG31050.038.899893
AACGCAG32150.037.7151266
GTACATG61100.035.7034261
ACATGGG61700.034.58183
TACATGG63550.033.9570772
ACGCAGA38250.031.700437
GTATTAC1807.2759576E-1231.3430961
TATCAAC38700.031.2216112
CGCAGAG39500.030.6972488
CATGGGT15700.027.8394184
CATGGGG33300.027.5211934
TATACCG1205.8451496E-627.4153425
GTACGGT700.00882248526.865511
GGTATCA9650.026.7959121
GCAGAGT47450.025.5540879
CATGGGA22650.025.3144514
ATGGGTA11200.024.338115
Produced by FastQC (version 0.11.2)