FastQCFastQC Report
Sun 14 Apr 2019
SRR6575608_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575608_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717533
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC91360.3361872698510009No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT64890.2387827489123407No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA45570.16768885603229106No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC45370.16695289440827396No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA41080.15116651757310767No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT40730.1498785847310778No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT37430.137735217934796No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT36670.1349385637635311No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA33490.12323677394165959No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG32780.12062411017639896No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT31300.11517799415867258No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC30630.11271252271821539No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA29890.1099894647093522No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC29780.10958468581614281No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC27330.10056915592193362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50550.042.9144941
CAACGCA50050.039.809465
ATCAACG51750.038.41093
GTACATG85550.033.9932481
TACATGG85100.033.741792
ACGCAGA61450.032.424147
ACATGGG88100.032.377053
CGCAGAG64550.030.9619968
GTACTAG20150.030.5928881
ACCTAAG11150.030.3865831
TATCAAC71900.030.3281232
CTAACGC10550.029.8433023
CATGGGG68150.029.6501624
AACGCAG72250.029.5280346
ATGGGGG46050.028.6748525
TAGTACT33000.027.9104374
TCTAACG11150.027.8179742
GCAGAGT71800.027.7701139
TTAGGTA5650.027.4466924
GTACTGT35200.027.3670316
Produced by FastQC (version 0.11.2)