FastQCFastQC Report
Sun 14 Apr 2019
SRR6575608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717533
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC95740.3523048294169749No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT66530.24481763422928077No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA49340.18156173264501296No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC48770.1794642420165643No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA41760.15366878709476572No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT41580.15300642163315034No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT40160.14778109410262913No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT38200.14056867018726174No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA37830.13920714118283017No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG36670.1349385637635311No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC33560.12349436051006556No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT33170.12205923534323226No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA31220.11488360950906576No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC30210.11116700330777952No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT28830.1060888681020617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47500.044.1358071
CAACGCA50000.038.716245
ATCAACG51000.038.049933
GTACATG82850.032.963511
TACATGG83100.032.7506072
GTACTAG20150.031.4926011
ACATGGG85700.031.4706023
GTACTAA902.5930962E-531.3370821
TAGTACT33300.031.1832944
ACGCAGA62750.031.074237
ACCTAAG9100.030.4761731
AACGCAG69850.030.000916
CGCAGAG65550.029.6751988
GTACTGT36350.029.2125856
CTGTGCG36950.028.4839089
CATGGGG65750.028.3706134
TATCAAC73200.028.2542252
ATGGGGG43450.028.223885
AGGTATA4950.027.5269936
GCAGAGT71300.027.282049
Produced by FastQC (version 0.11.2)