FastQCFastQC Report
Sun 14 Apr 2019
SRR6575604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401070
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10570.2635450170793128No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6260.15608247936769143No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5720.14261849552447203No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5100.12715984740818312No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4890.12192385369137557No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4800.11967985638417233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.11718652604283543No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4220.10521854040441818No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4170.10397187523374972No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4150.10347320916548233No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4140.10322387613134865No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4040.10073054579001171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12900.038.247441
CATGGGT6200.037.8902054
ATGGGTA3900.037.3461385
ACATGGG27500.032.4615753
TACATGG27750.032.0038072
GTACATG28100.031.7724061
GGTACCT4000.031.7141028
CTAGAAC1351.3271347E-831.322573
TCAACGC16800.029.3649084
AACGCAG17100.029.1244956
CAACGCA17100.028.8497355
ATCAACG17150.028.7656273
ATGGGAG4600.028.5988675
TGGGTAC4900.025.8890636
GGGTACC4900.025.8890637
ACGCAGA19250.025.8716327
GGTATCA7150.025.6307541
CATGGGG12750.025.4265584
CGCAGAG19600.025.4096378
TATCAAC19450.025.3671952
Produced by FastQC (version 0.11.2)