FastQCFastQC Report
Sun 14 Apr 2019
SRR6575595_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575595_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731365
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA61220.8370649402145304No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC32800.44847647891271797No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC26920.3680788662295844No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC22490.30750719544960453No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC20310.27769991727796656No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14410.19702884332720325No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA12890.17624578698734558No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT12230.1672215651555653No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG12080.1651706056483425No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11240.15368523240789483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10590.1447977412099294No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10550.14425081867466996No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC10530.14397735740704026No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC10280.14055909156166893No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10280.14055909156166893No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA10180.1391917852235204No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA10040.13727755635011246No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA9840.13454294367381542No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9250.12647583627873907No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG8880.12141680282758952No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA8730.11936584332036672No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG8610.11772507571458847No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT8550.11690469191169936No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT8520.11649450001025478No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8040.10993142958714186No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA7900.1080172007137339No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA7740.10582951057269627No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA7540.1030948978963992No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT7430.10159086092443581No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG7410.10131739965680611No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT7380.10090720775536154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4100.061.9553071
AAGGGTA3150.052.213225
GTATCAA20300.049.3573881
ATCAACG21350.045.341133
TCAACGC21700.044.826374
TATCAAC21850.044.5216832
CAACGCA21900.043.987855
GGTAATC4050.042.960248
AACGCAG22450.042.9101946
GTGCAAG5300.041.7148441
AGGGTAA4050.040.6102836
CTTAGGT9750.037.111553
TAGGTAT9500.037.098875
AGGTATA9650.036.52226
CAAGGGT5150.036.498564
ACGCAGA26750.036.012487
GTCTTAG10600.035.9457661
GTAATCA5950.035.5644079
GTACATG37700.035.436071
TTAGGTA9950.035.421034
Produced by FastQC (version 0.11.2)