FastQCFastQC Report
Sun 14 Apr 2019
SRR6575595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731365
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA53920.7372515775296876No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC35010.47869394898580053No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC27050.3698563644691775No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC27030.3695829032015478No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC20930.2861772165744874No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18730.25609647713521977No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA18100.24748244720488402No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA14690.20085730107401914No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC13090.17898039966364263No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT12450.17022963909949204No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT12070.16503387501452763No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10780.1473956232524116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.1471221619847819No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC10030.1371408257162976No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA9970.13632044191340847No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT9450.12921044895503614No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9360.12797987325070245No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA9130.12483506867296083No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9120.12469833803914598No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA9110.12456160740533112No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG9040.12360449296862715No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC8710.119092382052737No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT8470.11581084684118054No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8360.11430680986921715No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG8270.11307623416488347No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG8150.11143546655910525No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA8060.11020489085477156No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT7420.10145413029062095No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT7410.10131739965680611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4950.075.0251
GTATCAA23600.048.403681
TATCAAC24150.046.3280452
ATCAACG24400.045.6388553
CAACGCA24650.045.175995
TCAACGC24750.044.993464
AACGCAG25150.044.0910266
GTACTAG652.779725E-643.3932341
AAGGGTA5750.040.040965
GTGCAAG7250.037.6074681
TTAGGTA11150.037.0837824
ACGCAGA30000.036.962987
AGGTATA11250.036.754156
AGGGTAA6150.036.6726576
TAGGTAT11150.036.6623765
GTCTTAG13050.036.3827061
GGTAATC6200.036.376918
CTTAGGT11400.036.2705463
CGCAGAG30900.035.8863878
GCAGAGT32450.034.461849
Produced by FastQC (version 0.11.2)