FastQCFastQC Report
Sun 14 Apr 2019
SRR6575594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789939
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA57810.7318286601876854No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC35510.44952838130539197No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29450.372813596999262No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC27590.34926747508351913No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC22020.278755701389601No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA19670.2490065688616463No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG19420.24584176752888515No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA15800.20001544423050385No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC13010.16469626135688958No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT12980.16431648519695824No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT12410.15710073815826284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.1465935977334959No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA11290.14292242818749296No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC10890.13785874605507514No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA10610.13431416856238265No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10460.13241528776272599No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG10050.12722501357699773No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9770.12368043608430525No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA9370.11861675395188742No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA9270.11735083341878297No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA8910.11279351949960692No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT8910.11279351949960692No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT8650.10950212611353534No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA8550.1082362055804309No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG8430.10671710094070555No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC8390.10621073272746376No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG8190.10367889166125485No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT8060.10203319496821907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4650.063.6926041
CCCCGGT250.005235742356.3991666
AAGGGTA5600.046.160035
GTATCAA24100.045.25561
ATAGGGT751.4803663E-743.8660163
GGTAATC5950.043.4447368
GTGCAAG7450.042.9095571
GGGACCG450.001013836841.7771577
CAACGCA25400.041.4482045
TCAACGC25200.041.4041524
ATCAACG25250.041.322163
AGGGTAA6400.041.124396
TATCAAC26000.040.6827742
AACGCAG26850.039.7349786
GGGTAAT6750.038.295737
TAGGTAT10800.035.6846585
AGGTATA10700.035.578916
TTAGGTA10750.035.413434
CTTAGGT10750.035.413433
CAGTCCG801.1583086E-535.2494779
Produced by FastQC (version 0.11.2)