FastQCFastQC Report
Sun 14 Apr 2019
SRR6575593_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575593_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447734
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9420.2103927778547084No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6140.13713499533204984No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5870.1311046290878066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.11904389659932013No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4910.10966332688605288No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4720.10541973582528913No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4590.10251622615213497No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4590.10251622615213497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13700.045.763731
CATGGGT7250.037.6067474
GGTACCT4950.036.0792248
CAACGCA17200.035.8029375
AACGCAG17150.035.6332136
ATCAACG17500.035.457793
TCAACGC17200.035.2563254
GTACATG27450.034.8613321
TCGCCCG550.002715830234.18031794
TACATGG27700.033.7714042
ATGGGTA5200.033.4483075
GGGTACC5250.033.1223537
ACATGGG28200.032.505833
ACGCAGA19900.030.7021687
CGCAGAG20350.030.023258
TATCAAC21000.029.7720072
TGGGTAC5850.029.7318296
GTACTAT650.00606142129.009281
GTACCTG6350.028.1247469
CATGGGG12650.027.4990064
Produced by FastQC (version 0.11.2)