FastQCFastQC Report
Sun 14 Apr 2019
SRR6575593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447734
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10970.24501154703462324No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6940.15500274716684462No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5830.13021124149606686No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5530.12351083455801884No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5490.12261744696627909No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.1174804683137756No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4950.11055671447779263No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4730.10564308272322405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11950.051.1392441
ATCAACG15350.038.884563
TCAACGC15450.038.6328854
AACGCAG15350.038.2722056
CAACGCA15700.038.0177085
GTACATG26200.036.0639761
TACATGG27100.035.039752
ACATGGG27400.033.6191983
ACGCAGA18150.032.3679547
TATCAAC18350.032.27852
CGCAGAG18600.031.8375388
GCAGAGT21550.027.6973579
CATGGGG12600.027.6021584
CATGGGA11150.026.9765854
TAACCTT700.00883195426.8561525
CATGGGT6550.026.548644
ATGGGTA4700.025.9990435
ATGGGAG4200.025.7371465
GGGTACC4700.022.9991537
AGTACTG2154.9305527E-821.8596615
Produced by FastQC (version 0.11.2)