FastQCFastQC Report
Sun 14 Apr 2019
SRR6575592_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575592_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589482
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13270.2251128957287924No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7450.12638214568044487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7060.11976616758442157No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7030.11925724619241979No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6360.10789133510437977No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6350.10772169464037919No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6100.10348068304036424No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6000.1017842784003583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12750.044.3828541
GTCTTAG2850.038.056351
CGTACCC250.001701909137.6006142-43
TAGACGG500.001701927937.597425
GTATAGT2050.036.680418
GTACATG29050.036.3618551
TACATGG29150.036.2938582
TAGGTAT2100.035.8070685
TTAGGTA2000.035.247584
ACATGGG30400.034.0108263
ATCAACG17100.033.2550283
CAACGCA17200.032.515215
AACGCAG17200.032.515216
TCAACGC17600.032.3102844
TAACGCT600.00416074931.3311824
CTTAGGT2400.031.3311823
GGTACCT5850.030.527828
CATGGGG16200.030.4608754
TCTTAGG3600.030.0410062
GTACTTG3800.029.783231
Produced by FastQC (version 0.11.2)