FastQCFastQC Report
Sun 14 Apr 2019
SRR6575590_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575590_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613659
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA82510.5113224045476771No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC54180.3357586702023166No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC32790.2032027832398295No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG20690.12821791964721171No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT20150.12487148771828498No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC20140.12480951675663818No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC20080.12443769098675743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26350.052.2381671
GGTATCA8800.049.8179661
ATCAACG29600.045.4171643
TCAACGC30250.044.593884
CAACGCA31850.042.3536835
TATCAAC32600.041.237672
AACGCAG32900.041.0019766
TTAGGTA14400.034.9252934
TAGGTAT14400.034.9252935
AGGTATA14350.034.7194446
ACCTAAG22100.033.91491
ACGCAGA40250.033.393747
GTACTAA1001.3793051E-632.9977071
CTTAGGT15000.032.9036253
GTCTTAG17550.032.5007931
CTAAGAC25250.031.0885723
GTACATG61050.030.808671
CGCAGAG43900.030.7243218
TAAGACA29700.030.7018154
TACATGG61550.030.5476362
Produced by FastQC (version 0.11.2)