FastQCFastQC Report
Sun 14 Apr 2019
SRR6575590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613659
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA81260.5035760343418281No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC55170.341893795405349No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC32640.2022732188151276No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG22980.14240926986432698No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC22400.13881495408881306No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC20340.126048935989574No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT19840.12295038790723442No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT17790.11024634076964217No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA17280.10708582172565578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6900.049.06641
GTATCAA25900.049.0190351
TAAATCG205.679057E-446.99865316-17
TCAACGC27450.044.858464
ATCAACG28000.044.145163
CAACGCA28650.042.979575
AACGCAG29600.042.0764966
TATCAAC30350.041.057072
GTCTTAG16550.038.3562851
TTAGGTA14500.037.274794
AGGTATA14500.036.9506656
CTTAGGT14700.036.4479333
TAGGTAT14900.036.2741245
ACCTAAG23250.034.179411
GTATTCG851.7555629E-533.175529
GGTATAG16000.032.8990557
TACATGG54750.032.7221342
CTAAGAC26550.032.5715683
GTACATG54700.032.3222271
TCTTAGG19150.031.1842782
Produced by FastQC (version 0.11.2)