FastQCFastQC Report
Sun 14 Apr 2019
SRR6575581_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575581_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335446
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8560.25518265234940946No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5180.15442127794041366No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5120.1526326144893664No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4740.14130441263273372No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4630.13802519630581375No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4610.13742897515546468No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4570.1362365328547665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.12222533582156295No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3970.11834989834429387No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3790.11298390799115209No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3720.11089713396493027No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3660.109108470513883No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3630.10821413878835938No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3580.10672358591248667No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3520.10493492246143939No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3440.10255003786004305No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3440.10255003786004305No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3440.10255003786004305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCT2950.035.0704888
GTACATG24400.033.5351751
CATGGGG8550.033.5259444
ATGGGTA3650.032.1858065
ACATGGG24950.032.0181053
TACATGG25850.031.4487082
GATATAA902.5785976E-531.3508911
TAATACC1052.0625885E-631.327524
CATGGGT5300.031.0319754
GTATCAA14750.030.9257931
ATGGGAG3700.030.480835
GGGTACC3600.030.0222057
ATGGGGG3350.029.4572185
AACGCAG16200.027.8466826
ATCAACG17000.026.5362513
CAACGCA17200.026.227695
GTAGTGT909.513881E-426.1062646
TCAACGC17400.025.9262224
GGGTATC1301.0801303E-525.3218739
ACGCAGA18100.024.9235527
Produced by FastQC (version 0.11.2)