FastQCFastQC Report
Sun 14 Apr 2019
SRR6575581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335446
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9940.2963219117234965No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5820.17350035475158446No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5390.16068160001907908No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5390.16068160001907908No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5200.15501749909076276No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5060.15084395103831913No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5030.1499496193127955No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4630.13802519630581375No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4140.12341777812226111No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4020.11984045122016658No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3910.11656123489324659No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3750.11179146569045391No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3740.11149335511527936No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3730.1111952445401048No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG3660.109108470513883No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3640.10851224936353392No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT3440.10255003786004305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.10165570613451941No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3390.10105948498417033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA1204.2000465E-935.239664
GTACATG24250.035.070121
ACATGGG24400.034.469393
CATGGGG10150.034.255964
TACATGG25950.032.7726552
ATGGGCG753.2762677E-431.324145
AGAGTAT753.2762677E-431.324146
CATGGGT4850.030.0324234
ATGGGAT2200.029.9003145
ACACAAT804.7819587E-429.366381
ACCTAAC650.006156860428.914598
ATACATC650.006156860428.914596
ATGGGTA3550.027.794665
GTGTTAG1205.840333E-627.4086251
ATGGGGA5350.027.2256555
CGACGGT1406.3620246E-726.8492647
GTAGGTG700.00883990326.8492645
TATAGGG700.00883990326.8492642
CTTAGTG700.00883990326.8492643
CTATAGA909.519872E-426.103451
Produced by FastQC (version 0.11.2)