FastQCFastQC Report
Sun 14 Apr 2019
SRR6575580_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575580_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097731
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA85090.7751443659694406No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC52250.4759818206828449No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC33450.30471946223619445No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG23300.21225600807483802No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC20160.18365155033428043No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC19360.17636379040038042No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA15780.14375106469617782No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT15130.1378297597498841No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC14910.13582562576806156No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT14590.13291052179450158No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG14390.13108858181102656No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT14120.1286289628333353No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG13340.12152339689778278No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA12990.11833500192670154No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC12830.11687744993992154No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA12700.11569318895066277No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12360.11259589097875526No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA12260.11168492098701777No Hit
TCCAGGAACTGTGCAAAGTCATCCATGACAGTCTTCAGTTGCTCCGCTGT11900.10840542901676277No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT11860.10804104102006776No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT11180.10184644507625275No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG11080.10093547508451524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6600.061.3284951
GTATCAA23650.048.7576521
TATCAAC27600.041.374422
ATCAACG27750.040.8120843
TTAGGTA11550.040.686724
AGGTATA11050.040.4013676
TCAACGC28500.039.902974
TAGGTAT11250.039.683125
CAACGCA28800.039.4873125
CTTAGGT12000.039.1609733
AACGCAG29850.038.4131746
GTCTTAG14650.037.9098631
GGTATAG12050.037.438547
GTATAGT13400.035.4428258
GGTAATC4400.035.2673658
TATAGTA13600.034.5758489
GTACTAG851.7379256E-533.22311
TCTTAGG16350.033.05332
AAGGGTA4800.032.3078045
GTACATG57750.032.273871
Produced by FastQC (version 0.11.2)