FastQCFastQC Report
Sun 14 Apr 2019
SRR6575570_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575570_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370402
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8930.24108941096430367No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6270.16927554386855362No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6150.1660358205409258No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5460.14740741140706584No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5330.14389771113546904No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4770.12877900227320588No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4160.11231040869109778No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4080.11015059313934589No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.108530731475532No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3910.10556098509187316No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3890.10502103120393518No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3830.10340116954012128No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3750.10124135398836939No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3750.10124135398836939No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGTT250.001701961237.5985452-53
GTATCAA15050.033.7855571
GTGTTAT1001.3889749E-632.956561
CATGGGT5150.031.940514
GCCGAAA753.2720345E-431.33211794
GGTACCT3200.030.8425548
AACGCAG16650.029.9207636
CAACGCA16750.029.7421285
AAGACCG953.7464793E-529.6830625
GTACATG25650.029.551691
ATCAACG17050.028.943163
TCAACGC17150.028.7743974
ACATGGG26000.028.7411923
TACATGG26350.028.5377942
ATGGGTA3800.028.4462665
ATGGGGA5900.027.8802745
ATAGACG856.80885E-427.6459878
CATGGGG10700.027.2325884
TATCCGC350.008835726.85610452-53
GGGTACC3850.026.85617
Produced by FastQC (version 0.11.2)