FastQCFastQC Report
Sun 14 Apr 2019
SRR6575570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370402
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10750.2902252147666589No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6260.16900556692458465No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5720.15442681195025945No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5660.15280695028644553No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5110.13795821836815136No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4860.13120879476892672No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4650.12553927894557804No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4610.1244593711697021No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4450.12013974006619835No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4220.1139302703549117No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4160.11231040869109778No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4120.11123050091522184No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4040.10907068536346998No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3880.1047510542599662No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG3870.10448107731599722No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3840.10367114648409026No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT3830.10340116954012128No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3830.10340116954012128No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3770.10178130787630735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCGG1302.2919266E-1036.1537445
CGTATAG550.002712883834.1863371
GTACATG24050.032.8359381
TACATGG24450.032.2987442
ACATGGG23950.032.1836243
ATGGGAT1501.0641088E-931.3332445
ATAGTGC753.2713445E-431.3332443
ATGGGTA3800.028.4472875
CATGGGG9750.028.4409434
ATGGGCG1005.3140382E-528.1999195
CATGGGT6350.028.1259044
CTCTAGG2104.9112714E-1126.8606912
GTTGTCG700.00882855426.85706994
GTATCAA13500.026.810951
GGTACCT3050.026.1966468
ATGGGGA6500.026.0306935
TAGGACA1201.834809E-423.4999354
CACTCTA3550.022.5069779
TCTAGGC2304.0763553E-922.4781973
CCTAAGA1050.002330741822.383912
Produced by FastQC (version 0.11.2)