FastQCFastQC Report
Sun 14 Apr 2019
SRR6575565_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575565_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018905
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA181711.7833851045975828No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC135451.3293682924315808No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC93580.9184369494702647No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC48460.47560861905673246No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG47560.46677560714688804No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT41610.408379583965139No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG41430.40661298158317016No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC37750.37049577732958416No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT37560.3686310303708393No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA33930.3330045490011336No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT33760.3313360911959407No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG33730.3310416574656126No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA33220.3260362840500341No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT31550.30964613972843397No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA30770.30199086273990217No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA30420.29855580255274045No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC30300.2973780676314279No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT30070.2951207423655787No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA27360.2685235620592695No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT27280.2677384054450611No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA26260.2577276586139041No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT25860.2538018755428622No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT24720.24261339379039265No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC24530.2407486468316477No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC24430.23976720106388721No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG23960.2351544059554129No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA22130.217193948405396No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA22040.21631064721441157No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA21860.2145440448324427No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT20960.2057110329225983No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC20490.20109823781412398No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCACCTCATTAACGAAA20300.19923349085537906No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG20260.19884091254827488No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA20190.1981539005108425No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA19590.19226522590427958No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT19180.1882412982564616No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA18660.18313778026410704No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT18380.1803897321143777No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA17300.16979011782256442No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT17100.16782722628704344No Hit
GTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT17070.1675327925567153No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG16830.1651773227140901No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC16630.16321443117856915No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT16550.16242927456436076No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG16540.16233112998758473No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT16480.16174226252692842No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC16220.15919050353075115No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG16200.15899421437719904No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC16150.15850349149331883No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG16140.15840534691654276No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC16090.15791462403266252No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG16080.15781647945588645No Hit
GGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGC15900.15604987707391757No Hit
CTCTTACAGTATGGAATTTAAGAACTAAGGAATTCTCCAAGACATGGATT15810.15516657588293314No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT15740.15447956384550082No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA15730.15438141926872476No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT15700.15408698553839661No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA15590.15300739519386009No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA15530.1524185277332038No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA15460.15173151569577145No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC15410.15124079281189118No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC15310.1502593470441307No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA14380.14113190140395818No Hit
TAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTT14310.14044488936652583No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC13750.13494879306706709No Hit
GAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAG13740.13485064849029105No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGAACATGGCAAC13670.1341636364528587No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC13650.13396734729930662No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT13640.13386920272253056No Hit
CCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAAC13590.1333784798386503No Hit
ACCATAGATAATTCGGAGCACTCTTCATCTCTTACAGTATGGAATTTAAG13310.13063043168892094No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA13200.1295508413443844No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG13160.12915826303728023No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC13130.12886382930695206No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG13080.12837310642307184No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC13050.12807867269274367No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG12940.12699908234820714No Hit
CCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCAGAC12660.12425103419847777No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA12600.12366216673782147No Hit
CTCTTCATCTCTTACAGTATGGAATTTAAGAACTAAGGAATTCTCCAAGA12380.1215029860487484No Hit
GTATCAACGCAGAGTACATGGGGGAGTGTAGCCACGATCACAAGAAAGAC12120.11895122705257113No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC11730.11512358855830525No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA11640.11424028736732081No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT11200.10992192598917466No Hit
ATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTG11010.10805717903042973No Hit
TTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATT11000.10795903445365368No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA10940.1073701669929974No Hit
ATTCCATACTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTG10920.10717387783944529No Hit
CTCCAAGACATGGATTTGCTCCAGAAAAAGTCTAAAGTTGCCATTATCTT10830.10629057664846085No Hit
GTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG10470.1027573718845231No Hit
GTCTTGGAGAATTCCTTAGTTCTTAAATTCCATACTGTAAGAGATGAAGA10450.102561082730971No Hit
GGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGA10380.10187407069353865No Hit
GGCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAAT10380.10187407069353865No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT10320.10128520323288234No Hit
AGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGG10260.10069633577222607No Hit
CATTAACGAAAAGGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCC10260.10069633577222607No Hit
CTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACCTTCCAGC10200.10010746831156977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5000.071.565441
GGTAATC6450.047.3869328
GTGCAAG6850.046.051521
GTATCAA29700.045.4972531
ATCAACG29000.045.2099463
TATCAAC29050.045.134352
AGGGTAA6950.044.6258966
TCAACGC29300.044.5866624
CAACGCA29900.044.006285
AAGGGTA6950.043.9497455
AACGCAG30800.042.872956
GGGTAAT7400.041.2771267
TGCAAGG7750.040.0213172
GTAATCA8400.039.1853459
GTACTAG853.901023E-738.7738461
ACTAGGC1003.3311153E-837.5939373
TTAGGTA33450.036.94774
GTCTTAG34250.036.8412171
TAGGTAT33900.036.5958675
ACCTAAG53050.036.4767531
Produced by FastQC (version 0.11.2)