FastQCFastQC Report
Sun 14 Apr 2019
SRR6575565_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575565_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018905
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA170291.6713039979193351No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC136671.3413419307982588No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC89150.874958901958475No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG54830.5381267144630756No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC52010.5104499438122299No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG42960.42162910182990565No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT40490.39738739136622153No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA38570.37854363262522017No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT37820.37118278936701654No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC36940.3625460666107243No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT33230.32613442862681014No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC32950.3233863804770808No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA31910.3131793444923717No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA31230.3065055132716004No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT31210.3063092241180483No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT28790.28255823653824447No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA28600.2806934895794996No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT27840.27323450174451985No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT27720.2720567668232073No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA27110.2660699476398683No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT26700.2620460199920503No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA24580.24123936971552792No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG24330.23878575529612672No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC23020.22592881573846435No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA22730.2230826230119589No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA22110.2169976592518439No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA22090.21680137009829178No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT20240.19864462339472277No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG19950.19579843066821734No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC19660.19295223794171193No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA19530.1916763584436233No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT19410.1904986235223107No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA18960.18608211756738852No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG18930.18578768383706037No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA18800.18451180433897174No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT18410.18068416584470584No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCACCTCATTAACGAAA18300.17960457550016928No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG18070.1773472502343202No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC17790.17459920208459082No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA17610.17283259970262194No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC17410.17086970816710095No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG16980.16664949136573085No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG16850.1653736118676422No Hit
GTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT16820.1650791781373141No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT16820.1650791781373141No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC16370.16066267218239189No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT16250.1594849372610793No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT15660.15369440723129243No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT15630.15339997350096427No Hit
GGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGC15460.15173151569577145No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA15430.1514370819654433No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC15430.1514370819654433No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG15310.1502593470441307No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA15190.14908161212281812No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC15110.14829645550860973No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA15030.14751129889440134No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC15000.14721686516407317No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC14950.14672614228019296No Hit
CTCTTACAGTATGGAATTTAAGAACTAAGGAATTCTCCAAGACATGGATT14870.14594098566598457No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA14830.14554840735888036No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT14470.1420152025949426No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC14410.14142633513428632No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGAACATGGCAAC14360.14093561225040607No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA14200.1393652990219893No Hit
TAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTT14120.1385801424077809No Hit
GAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAG13880.13622467256515575No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG13770.1351450822206192No Hit
ACCATAGATAATTCGGAGCACTCTTCATCTCTTACAGTATGGAATTTAAG13480.13229888949411378No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC13450.13200445576378564No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT13130.12886382930695206No Hit
CCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAAC13100.12856939557662392No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA12960.12719537150175925No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA12850.12611578115722272No Hit
CTCTTCATCTCTTACAGTATGGAATTTAAGAACTAAGGAATTCTCCAAGA12750.12513433538946223No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG12460.12228814266295679No Hit
GGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGA12430.12199370893262865No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG12330.12101226316486816No Hit
ATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTG12140.11914751620612325No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC12040.11816607043836276No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT11990.11767534755448252No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG11600.11384770906021661No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC11500.11286626329245612No Hit
TCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATG11430.11217925125502379No Hit
GGGTATATCAAAGAAAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT11380.11168852837114354No Hit
GTATGATGGATTCAATACATTTACTATACCTAAGACAGACTATGATAACT11330.1111978054872633No Hit
CAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTG11250.11041264887305491No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT11200.10992192598917466No Hit
CCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCAGAC11030.10825346818398184No Hit
CTCCAAGACATGGATTTGCTCCAGAAAAAGTCTAAAGTTGCCATTATCTT11020.10815532360720578No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT10940.1073701669929974No Hit
GTATCAACGCAGAGTACATGGGGGAGTGTAGCCACGATCACAAGAAAGAC10790.10589799834135666No Hit
AATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCC10780.1057998537645806No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA10650.10452397426649197No Hit
AGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGG10600.10403325138261173No Hit
GTCTTTGATTCTTGATGAGGTATCCTAGGTAACATGTGGATAGACTGGAA10530.10334623934517939No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC10380.10187407069353865No Hit
CCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTT10300.10108891407933027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6800.069.115341
AAGGGTA7050.051.3215375
AGGGTAA7700.047.599456
GTGCAAG7950.044.9293171
ATCAACG26750.041.9828763
CAACGCA27600.041.200685
TCAACGC27500.041.179634
TATCAAC27500.040.8479162
AACGCAG28250.040.5853656
GGTAATC9000.039.6797758
TGCAAGG8300.039.6391752
TTAGGTA32400.038.7225844
CTTAGGT32500.038.603443
GTCTTAG33300.038.5302431
GTATCAA30300.038.467361
TAGGTAT32800.038.393625
AGGTATA33300.038.0993546
TCTTAGG33550.037.964832
GGGTAAT9500.037.5913667
ACCAGAT53150.036.86641394
Produced by FastQC (version 0.11.2)