FastQCFastQC Report
Sun 14 Apr 2019
SRR6575564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078613
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA171411.5891705366058075No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC134571.247620787066353No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC90340.8375571219705306No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG55470.5142715691355472No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC53950.5001793970590007No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG43000.3986601311128273No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT41560.3853096523034675No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT40260.37325713671168437No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA39120.3626880076542745No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC38660.3584232713679512No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT35460.328755540680485No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC33120.30706101261527535No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT32960.30557762608090205No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA32660.3027962763289521No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA31850.2952866319986872No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT29950.2776714169030041No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT29170.27043990754793423No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA29000.26886380935516263No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA28840.2673804228207893No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT28670.2658043246280177No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT26210.242997256662028No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA25020.23196456931262652No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC24730.22927593121907486No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG24060.22306425010638664No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA23260.2156473174345201No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA22440.20804496144585685No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA21360.198032102338837No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC20580.19080059298376711No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT20580.19080059298376711No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG19770.18329094865350223No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA19680.18245654372791725No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG19530.18106586885194226No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT18860.17485418773925404No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA18590.17235097296249907No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCACCTCATTAACGAAA18470.17123843306171907No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT18470.17123843306171907No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG18260.16929148823535412No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC18230.16901335326015912No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA18130.16808623667617578No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG17800.16502675194903083No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA17110.15862964751954595No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG17100.15853693586114762No Hit
GTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT16840.15612643274279098No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT16810.15584829776759598No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT16730.1551066045004093No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG16720.15501389284201098No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT16610.15399406459962933No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC16550.15343779464923935No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC16240.15056373323889108No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA16180.15000746328850106No Hit
GGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGC15960.14796780680373778No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA15760.14611357363577113No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC15540.1440739171510078No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC15510.14379578217581282No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA15430.14305408890862617No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC15420.14296137725022784No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC15080.13980918086468455No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC15060.1396237575478879No Hit
CTCTTACAGTATGGAATTTAAGAACTAAGGAATTCTCCAAGACATGGATT14870.1378622360383196No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT14620.1355444445783613No Hit
TAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTT14230.13192868990082635No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA14040.13016716839125803No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA13990.12970361009926637No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA13950.12933276346567305No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT13660.12664412537212144No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT13640.12645870205532478No Hit
GAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAG13520.12534616215454478No Hit
CCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAAC13380.12404819893696813No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGAACATGGCAAC13330.12358464064497646No Hit
GGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGA13160.12200854245220483No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC13010.12061786757622983No Hit
CAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTG12940.11996888596744151No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA12680.11755838284908489No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT12640.11718753621549156No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG12550.11635313128990658No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG12510.11598228465631326No Hit
GTATCAACGCAGAGTACATGGGGGAGTGTAGCCACGATCACAAGAAAGAC12400.11496245641393159No Hit
TCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATG12340.1144061864635416No Hit
ACCATAGATAATTCGGAGCACTCTTCATCTCTTACAGTATGGAATTTAAG12220.11329364656276163No Hit
CTCTTCATCTCTTACAGTATGGAATTTAAGAACTAAGGAATTCTCCAAGA12210.1132009349043633No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG12020.11143941339479499No Hit
AGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGG11850.10986331520202333No Hit
GGGTATATCAAAGAAAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT11800.10939975691003168No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC11760.10902891027643835No Hit
ATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTG11640.10791637037565838No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT11470.10634027218288672No Hit
GTATGATGGATTCAATACATTTACTATACCTAAGACAGACTATGATAACT11330.10504230896531007No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC11130.10318807579734345No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT11040.10235367087175844No Hit
AATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCC10930.1013338426293768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6650.068.5793151
AAGGGTA7350.048.596875
GTGCAAG7650.048.552181
ATCAACG30100.045.436883
TATCAAC31000.044.5891042
TCAACGC30850.044.48464
CAACGCA31700.043.5883145
AGGGTAA8200.042.9862376
AACGCAG32850.042.0623936
TTAGGTA35800.041.090684
GTCTTAG36050.040.820861
GTATCAA35350.040.565191
CTTAGGT36350.040.2103653
TAGGTAT36700.040.0830045
AGGTATA37050.039.7043576
TCTTAGG37300.039.326822
TGCAAGG9100.037.7159082
GGTATAG39150.037.5746157
GGGTAAT9350.037.1965037
TATAGTA40300.036.6190159
Produced by FastQC (version 0.11.2)