FastQCFastQC Report
Sun 14 Apr 2019
SRR6575562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140579
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA74780.6556319202790863No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC55070.4828249511870725No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC42430.37200404356033206No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC25310.221904839559557No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG21010.1842046890219792No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG18340.16079552578120412No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT16120.14133172713157088No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA14890.13054773058244978No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT14880.13046005581375775No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14820.1299340072016055No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT14180.12432282200531486No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT13380.11730884050995152No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13210.11581836944218682No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12890.11301277684404148No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC12610.11055788332066432No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG12580.11029485901458821No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT12230.10722624211036676No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT12130.10634949442344634No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA11980.10503437289306572No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA11550.10126435783930793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5550.058.4538881
GTATCAA21600.050.282341
ATCAACG22650.045.8574563
TCAACGC23200.044.770324
CAACGCA23750.043.7335365
AACGCAG24700.042.622316
TTAGGTA14950.042.4403044
TATCAAC25150.041.5023082
CTTAGGT15100.041.0849653
TAGGTAT15300.040.8550835
GTCTTAG16350.040.834561
AGGTATA15350.040.7220086
GGTATAG16150.038.4138037
TCTTAGG18050.036.728162
GTATAGT17350.035.7569438
TATAGTA17650.035.1491749
ACGCAGA30300.034.4346967
GGTAATC3900.033.7426648
ACCTAAG23750.033.2585221
CTAAGAC25250.032.014963
Produced by FastQC (version 0.11.2)