FastQCFastQC Report
Sun 14 Apr 2019
SRR6575548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159607
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2470.15475511725676191No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.11904239788981685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAT352.929837E-453.7142831
GTATCAA5000.039.481
GTATAAC500.001697639837.61
TTAATAC801.1524184E-535.2499963
GTACATG11350.034.3700451
TACATGG11350.034.3700452
ACATGGG11700.034.1452983
GTCTTAT702.1766163E-433.5714261
ATGGGAG2550.031.3333345
TCAACGC6450.031.3333324
ATCAACG6400.030.8437483
CAACGCA7100.029.788735
CATGGGA6000.029.7666654
AACGCAG7050.029.3333346
TATCAAC6950.028.4028782
CATGGGC1853.2196112E-1027.9459484
TGCTATA700.00881365626.8571412
TAGGCTG700.00881365626.8571415
CATGGGG4050.026.6913594
CGCAGAG8150.024.7975468
Produced by FastQC (version 0.11.2)