FastQCFastQC Report
Sun 14 Apr 2019
SRR6575547_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575547_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532923
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18940.3553984346706748No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14820.2780889546895142No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11430.21447751363705453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.19646365422396855No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9550.17920037228642788No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG8300.1557448261756389No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7650.1435479421980286No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC7440.13960741045141606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.13604216744257613No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6650.12478350530939741No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT6510.12215648414498906No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6270.11765301929171756No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA6240.11709008618505862No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA5920.11108546638069665No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA5900.11071017764292403No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT5820.10920902269183352No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG5410.10151560356749476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT200.002147454370.6228941
GTATCAA22500.056.707571
GGTATCA7050.050.0871621
GTACTAG3000.042.3737371
ATCAACG30300.041.0999873
CAACGCA30750.040.6513525
GTGCAAG1600.038.2540661
AACGCAG33700.037.9259726
GCTTAGG500.001686714137.6655431
TCAACGC33450.037.5105634
TATCAAC34850.036.007062
AAGGGTA1601.8189894E-1235.245185
ACGCAGA36000.034.7197727
ACCTAAG2600.034.4060251
TAGTACT5200.034.3414544
CGCAGAG36650.033.9981238
TAGGCAT4150.032.8388825
GCATCGT4050.032.5076838
CCTAAGA3050.032.3592642
GTACATG36600.032.288431
Produced by FastQC (version 0.11.2)