FastQCFastQC Report
Sun 14 Apr 2019
SRR6575546_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575546_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559051
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19930.356496992224323No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14980.26795408647869334No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12630.22591856556915202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10570.18907040681440512No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10000.17887455706187808No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG8670.1550842409726483No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA8340.14918138058960634No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8230.14721376046192566No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT7480.1337981686822848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6930.12396006804388152No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA6810.12181357335913896No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6780.12127694968795334No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6650.11895158044614891No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6560.117341709432592No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6120.10947122892186938No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT5810.10392611765295116No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA5670.10142187385408485No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG5660.10124299929702299No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA5660.10124299929702299No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC5600.10016975195465173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23200.058.0869521
GGTATCA7100.051.7650681
GTACTAG3200.045.6471
ATCAACG31000.042.0056043
GTGCAAG2250.041.8839871
CAACGCA31250.041.6658335
TATCAAC33400.040.5354732
TCAACGC33850.038.6043664
CCCCGTA500.001699523137.607912
GGTAATC2000.037.5978168
AACGCAG35950.037.2645876
ACGCAGA38000.034.3822147
ATTAGAC550.00271302134.1890073
CGCAGAG38600.033.847788
TAGTACT6150.033.630084
GTACTGT6400.033.050876
TATATAC1001.407132E-632.906923
CTGTGCG6300.032.823499
CTAGTAC6650.032.5181163
AAGGGTA2350.032.003875
Produced by FastQC (version 0.11.2)