FastQCFastQC Report
Sun 14 Apr 2019
SRR6575537_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575537_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347385
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.15170488075190353No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4950.14249319918822057No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3690.10622220303121896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG250.00521817356.4403794
ACCGTAT250.005221149456.4322368
GTATCAA10750.043.333431
GTACATG20850.038.591031
TACATGG20700.038.1392632
ACATGGG21450.036.3675583
CATGGGG7850.035.319594
AACGCAG13000.033.6180846
ATCAACG13650.032.3614923
CAACGCA13650.032.0172235
GTACTAG753.2489252E-431.3693181
ATTATGC753.273725E-431.328683
TCAACGC14250.030.6691284
TATCAAC15200.029.9889662
CGCAGAG15550.028.4278148
ACGCAGA15450.028.2870587
ATGGGAG5200.028.0150685
CATGGGA12550.026.5856914
TAGTACA1258.012097E-626.316094
GCAGAGT17750.024.904379
Produced by FastQC (version 0.11.2)