FastQCFastQC Report
Sun 14 Apr 2019
SRR6575537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347385
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5620.16178015746218172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.13529657296659325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10350.044.951841
TGCCCGC651.4123251E-436.150081
TGCACCG651.4135218E-436.144885
ACATGGG20600.035.8115043
TACATGG20750.035.552622
CATGGGT4500.035.50234
GTACATG21200.035.0246541
ACCGTGG550.002717714534.173348
ATCAACG13550.033.637413
TCAACGC13900.033.128474
AACGCAG13900.032.7904246
CAACGCA14300.032.2017975
TATCAAC14850.031.6419812
CGTTCGC300.00411235831.40693548-49
ATAAGGC753.2756358E-431.3255623
CGCAATA404.7137454E-429.44400450-51
TCTATAC804.7810352E-429.3677143
ACGCAGA16300.027.9623857
CATGGGA10800.027.8449424
CGCAGAG16400.027.7918838
Produced by FastQC (version 0.11.2)