FastQCFastQC Report
Sun 14 Apr 2019
SRR6575529_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575529_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986399
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA117281.1889711972538497No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC81170.8228921562167033No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC71730.7271905182385627No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC37400.3791569131761082No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG34420.34894601474656806No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG29910.3032241516870962No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT29260.29663452619072No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT27660.28041390958425544No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC27430.27808219594707617No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA24770.25111542083882893No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA24420.24756716095616482No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT24390.2472630243947936No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT24290.24624923585688957No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA23360.23682100245438203No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA22020.223236236046468No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA21310.21603833742734938No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC21090.2138080026439605No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT20470.2075225137089555No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG20090.20367011726492018No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA18760.19018672971079653No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT18650.1890715623191021No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT18520.18775363721982688No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA18050.1829888310916779No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT17770.18015022318554663No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA16520.1674778664617462No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG16110.16332133345633967No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC15420.15632619254480187No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG15350.15561654056826904No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC15270.1548055097379458No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA14940.1514600075628625No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14880.15085173444012007No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA14820.15024346131737765No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG13780.1397000605231757No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA13740.1392945451080141No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA13530.13716558917841565No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT12800.1297649328517162No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG12640.12814287119106973No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT12360.12530426328493843No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA12200.123682201624292No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT12060.12226289767122635No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC11640.11800498581202941No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG11540.11699119727412537No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCACCTCATTAACGAAA11450.11607878759001175No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11400.11557189332105974No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC11350.11506499905210772No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA11290.11445672592936529No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA11260.11415258936799408No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC11220.11374707395283247No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC11010.111618118023234No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC10980.11131398146186279No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA10870.11019881407016835No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA10860.11009743521637795No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA10850.10999605636258754No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT10820.10969191980121636No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG10770.10918502553226433No Hit
GTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT10720.10867813126331231No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC10660.1080698581405699No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG10660.1080698581405699No Hit
GAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAG10600.10746158501782747No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT10540.10685331189508504No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC10380.1052312502344386No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC10220.10360918857379214No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC10100.10239264232830732No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT10040.1017843692055649No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT9920.10056782296008004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5400.064.577361
GTGCAAG6900.048.4899371
AGGGTAA7350.046.0452886
GGTAATC7400.045.7272158
AAGGGTA7350.045.405775
GTATCAA24950.045.1408421
TATCAAC25850.042.5495832
ATCAACG26400.041.6631363
TCAACGC26450.041.5843774
CAACGCA27050.040.835765
AACGCAG27750.039.975056
TGCAAGG7900.039.2696342
TTAGGTA23900.038.154334
GTCTTAG24850.037.9267771
CTTAGGT24300.037.719713
GTAATCA9550.037.401089
AGGTATA24550.037.1441356
TCTTAGG25400.037.011472
TAGGTAT24750.036.8439835
GGGTAAT9150.036.4679377
Produced by FastQC (version 0.11.2)